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enzyme can bind but a single divalent cation inside the Energetic site. For just a binuclear steel Centre, the activation with the amide bond and the activation on the solvent h2o may be distributed independently between The 2 metal ions. Nonetheless, from the mononuclear metal Heart, each capabilities have to be borne by only one divalent cation. These benefits highlight the significant variety for your evolution of functionality within the amidohydrolase superfamily.
with a variety of substrate analogs. The reaction mechanism is of interest since NagA from bacterial sources is identified with either one or two divalent metallic ions during the Energetic web-site.
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